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Melatonin Ameliorates Busulfan-Induced Spermatogonial Stem Cell Oxidative Apoptosis in Mouse Testes

作者:Li, Bo;He, Xin;Zhuang, Mengru;Niu, Bowen;Wu, Chongyang;Mu, Hailong;Tang, Furong;Cui, Yanhua;Liu, Weishuai;Zhao, Baoyu;Peng, Sha;Li, Guangpeng;Hua, Jinlian 来源: ANTIOXIDANTS & REDOX... 收录类型: SCIE; SCOPUS; PubMed; - 被引量:4 - 年份:2018

Aims: Many men endure immunosuppressive or anticancer treatments that contain alkylating agents before the age of sexual maturity, especially the increasing number of preadolescent males who undergo busulfan treatment for myeloablative conditioning before hematopoietic stem cell transplantation. Before sperm production, there are no sperm available for cryopreservation. Thus, it is necessary to identify a solution to ameliorate the busulfan-induced damage of spermatogonial stem cells (SSCs). Results: In this study, we demonstrated that melatonin relieved the previously described SSC loss and apoptosis in mouse testes. Melatonin increased the expression of manganese superoxide dismutase (MnSOD), which regulated the production of busulfan-induced reactive oxygen species (ROS). Moreover, melatonin promoted sirtuin type 1 (SIRT1) expression. SIRT1 participated in the deacetylation of p53, which promotes p53 ubiquitin degradation. Decreased concentrations of deacetylated p53 resulted in spermatogonial cell resistance to apoptosis. Acute T cell leukemia cell assay demonstrated that melatonin does not affect busulfan-induced cancer cell apoptosis and ROS. Innovation: The current evidence suggests that melatonin may alleviate the side effects of alkylating drugs, such as busulfan. Conclusion: Melatonin promoted MnSOD and SIRT1 expression, which successfully ameliorated busulfan-induced SSC apoptosis caused by high concentrations of ROS and p53.

Ecological responses of Stipa steppe in Inner Mongolia to experimentally increased temperature and precipitation. 1: Background and experimental design

作者:Wan, Zhiqiang;Hu, Guozheng;Chen, Yali;Chao, Colmvn;Gao, Qingzhu 来源: RANGELAND JOURNAL 收录类型: SCOPUS; SCIE; - 被引量:6 - 年份:2018

Both temperature and precipitation are predicted to increase in the region of the Stipa steppe of Inner Mongolia. We conducted an experiment to simulate both the individual and combined effects of warming and increased precipitation on community structure (biomass and diversity) and ecological processes (soil respiration and ecosystem carbon exchange). Open-topped chambers were used to simulate climatic warming, and irrigation was applied to simulate a 20% increase in precipitation. Open-topped chambers were effective in producing significant increases in mean air temperature in each year of the experiment (2011-2015), with the average increase ranging from 2.5 degrees C to 3 degrees C in the final 3 years of the study. Mean soil temperature was significantly increased in 2 of the 5 years of observation. Irrigation significantly increased soil moisture. The experimental treatments thus produced environmental changes consistent with those expected in the region by mid-century and provide a basis for examining the likely effects of climate change on the grassland ecosystem as reported in companion papers.

Ecological responses of Stipa steppe in Inner Mongolia to experimentally increased temperature and precipitation. 5: Synthesis and implications

作者:Hu, Guozheng;Wan, Zhiqiang;Chen, Yali;Chao, Luomeng;Gao, Qingzhu;Wang, Xuexia;Yang, Jie 来源: RANGELAND JOURNAL 收录类型: SCOPUS; SCIE; - 被引量:3 - 年份:2018

A randomised block experiment was conducted to study the response of plant community characteristics (biomass, density and diversity) and ecosystem carbon exchange processes to warming, increased precipitation and their combination on Stipa steppe in Inner Mongolia. Increased precipitation enhanced the effect that warming had in promoting community diversity and biomass. Increased precipitation directly increased net ecosystem exchange and gross ecosystem productivity, although ecosystem respiration and soil respiration also increased. However, warming did not have a significant effect on net ecosystem exchange and gross ecosystem productivity, whereas ecosystem respiration and soil respiration were significantly decreased by warming. All carbon flux processes had a significantly positive correlation with soil moisture. However, the carbon sequestration processes, gross ecosystem productivity and net ecosystem exchange, were significantly negatively correlated with temperature, contrary to carbon emission processes, soil respiration and ecosystem respiration. Results suggest that Stipa steppe may be benefited by future climate change, as the predicted precipitation is increasing with warming in Inner Mongolia. However, it is hard to predict the feedback of Stipa steppe to climate, because of the uncertainty in magnitude and temporal dynamics of climate change. To reveal the mechanism of the observed responses, further studies are suggested in this region on the effects of altered climate variables on plant species interactions, soil organic carbon composition, soil extracellular enzyme activity, microbial biomass and microbial respiration.

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